Presentation
R for Bio Data Science - Group 8
Introduction: Paper and Background
Circulating Exosomal microRNAs as Predictive Biomarkers of Neoadjuvant Chemotherapy Response in Breast Cancer
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Neoadjuvant chemotherapy (NACT) |
an increasingly used approach for treatment of breast cancer |
Circulating Exosomal microRNAs(miRNA) |
small RNA molecules circulating in bodily fluids, potentially serving as biomarkers for treatment response after NACT |
Pathological Complete Response (pCR) |
absence of active cancer cells in a tissue from the tumor site after NACT |
Non-Pathological Complete Response (non-pCR) |
presence of active cancer cells in a tissue from the tumor site after the treatment |
Todorova, Valentina K., et al. “Circulating exosomal microRNAs as predictive biomarkers of neoadjuvant chemotherapy response in breast cancer.” Current Oncology 29.2 (2022): 613-630.
Our Goals of Study:
to compare gene expression and gene interaction networks for pre- and post- treatment
to conduct differential expression analysis across pCR and non-pCR groups
to investigate whether circulating exosomal microRNAs could predict pCR in breast cancer patients treated with NACT
Materials and methods: Flowchart
- potatoe
Methods: Metadata & Describe
Methods: Gene Expression Analysis
Methods: Virtual pull-down and Gene Enrichement Analysis
Results: Describe
Results: Gene Expression Analysis
Results: Virtual pull-down and Gene Enrichement Analysis
- After the pull-down we found:
- The nonpCR Group has 5 potential complexes or networks with upregulated or downregulated activity when comparing pre and post treatment
- The nonpCR Group has 11 potential complexes or networks with upregulated or downregulated activity when comparing pre- and post-treatment
- For each network the main enriched biological processes were identified